E-ISSN 2146-9369 | ISSN 2146-3158
 
2024, Vol: 14, Issue: 4
14 / 4Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article 


Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.

Swati Sharma, Deepshikha Bhowmik, Shiela Chetri, Deepjyoti Paul, Debadatta Dhar Chanda, Atanu Chakravarty, Amitabha Bhattacharjee.


Cited By:4

Abstract
Objective: The current study investigated the resistance against cycline group of antibiotic among clinical isolates of Staphylococcus aureus and molecular characterization with special reference to phenotypic screening of Tigecycline resistance.
Methods: 197 coagulase producing Staphylococcus spp. were collected from private diagnostic laboratories and Silchar Medical College and Hospital, India. Phenotypic screening was done with cycline groups of antimicrobials and genotypically characterized by PCR, targeting coa gene. Detection of tetK, tetM, tetO and tetX genes was performed by PCR. Antibiogram profiling was performed and clonality was also determined.
Results: 127 coagulase positive S. aureus were phenotypically screened with cycline group of antibiotics in which prominent resistant type showed highest non-susceptibility against Tigecycline 53.5% (n=68) while lowest against minocycline 20.5% (n=26). Multiplex PCR assay showed that tet(K) gene to be the most common cycline group of antimicrobial resistance determinant in S. aureus, followed by tet(M) gene. Multiple genes were also observed. However, resistant gene tet(O) was not reported. Antibiotic susceptibility testing and MIC for therapeutic purpose showed highest susceptibility rate against linezolid. DNA fingerprinting experimented with PFGE had shown seven pulsotypes.
Conclusion: Resistance to cycline group of antibiotics with special reference to Tigecycline is not frequently reported from India. Thus, this study is of clinical interest in terms of selection of proper antimicrobial chemotherapy and also using Tigecycline for phenotypic screening of cycline group of antibiotic resistance in Staphylococcus aureus. J Microbiol Infect Dis 2017; 7(4):173-177

Key words: Tigecycline, tet genes, Staphylococcus aureus, North-east India


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Swati Sharma
Articles by Deepshikha Bhowmik
Articles by Shiela Chetri
Articles by Deepjyoti Paul
Articles by Debadatta Dhar Chanda
Articles by Atanu Chakravarty
Articles by Amitabha Bhattacharjee
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Sharma S, Bhowmik D, Chetri S, Paul D, Chanda DD, Chakravarty A, Bhattacharjee A. Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.. J Microbiol Infect Dis. 2017; 7(4): 173-177 . doi:10.5799/jmid.368660

Web Style

Sharma S, Bhowmik D, Chetri S, Paul D, Chanda DD, Chakravarty A, Bhattacharjee A. Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.. https://www.jmidonline.org/?mno=302657173 [Access: January 09, 2025]. doi:10.5799/jmid.368660

AMA (American Medical Association) Style

Sharma S, Bhowmik D, Chetri S, Paul D, Chanda DD, Chakravarty A, Bhattacharjee A. Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.. J Microbiol Infect Dis. 2017; 7(4): 173-177 . doi:10.5799/jmid.368660


Vancouver/ICMJE Style

Sharma S, Bhowmik D, Chetri S, Paul D, Chanda DD, Chakravarty A, Bhattacharjee A. Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.. J Microbiol Infect Dis. (2017), [cited January 09, 2025]; 7(4): 173-177 . doi:10.5799/jmid.368660


Harvard Style

Sharma, S., Bhowmik, . D., Chetri, . S., Paul, . D., Chanda, . D. D., Chakravarty, . A. & Bhattacharjee, . A. (2017) Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.. J Microbiol Infect Dis, 7 (4), 173-177 . doi:10.5799/jmid.368660


Turabian Style

Sharma, Swati, Deepshikha Bhowmik, Shiela Chetri, Deepjyoti Paul, Debadatta Dhar Chanda, Atanu Chakravarty, and Amitabha Bhattacharjee. 2017. Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.. Journal of Microbiology and Infectious Diseases, 7 (4), 173-177 . doi:10.5799/jmid.368660


Chicago Style

Sharma, Swati, Deepshikha Bhowmik, Shiela Chetri, Deepjyoti Paul, Debadatta Dhar Chanda, Atanu Chakravarty, and Amitabha Bhattacharjee. "Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.." Journal of Microbiology and Infectious Diseases 7 (2017), 173-177 . doi:10.5799/jmid.368660


MLA (The Modern Language Association) Style

Sharma, Swati, Deepshikha Bhowmik, Shiela Chetri, Deepjyoti Paul, Debadatta Dhar Chanda, Atanu Chakravarty, and Amitabha Bhattacharjee. "Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.." Journal of Microbiology and Infectious Diseases 7.4 (2017), 173-177 . Print. doi:10.5799/jmid.368660


APA (American Psychological Association) Style

Sharma, S., Bhowmik, . D., Chetri, . S., Paul, . D., Chanda, . D. D., Chakravarty, . A. & Bhattacharjee, . A. (2017) Occurrence of tigecycline resistance in clinical isolates of Staphylococcus aureus from North East India.. Journal of Microbiology and Infectious Diseases, 7 (4), 173-177 . doi:10.5799/jmid.368660


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesPeer Review PolicyPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information

About Journal of Microbiology and Infectious Diseases

Journal of Microbiology and Infectious Diseases (JMID) is an international "peer review" journal in microbiology, infectious diseases, virology, ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Journal of Microbiology and Infectious Diseases

Department of Microbiology and Parasitology 

Faculty of Veterinary Medicine

University of Tripoli

P.O. Box 13662, Tripoli, Libya