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2024, Vol: 14, Issue: 3
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Research Article 


Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman.


Cited By:2

Abstract
Objectives: Entomotoxic microorganisms are becoming very effective as biocontrol agents in alternative to chemical formulations. This study aimed to evaluate isolation, characterization, and determination of the larvicidal activity of Bacillus thuringiensis against Culex and Aedes larvae.
Methods: Bacteria were isolated from soil samples collected from major flour mills in Ilorin metropolis. The isolates were screened biochemically to obtain B. thuringiensis, which isolated were further subjected to molecular characterization. Larva toxicity was determined against Culex and Aedes larvae using standard procedure. Four densities of bacilli load were prepared using the McFarland turbidity standard 0.5, 1, 2, and 3, and the time range was 0-48 hours.
Results: Five isolates were obtained and named BT1, BT2, BT3, BT4, and BT5. All the isolates were toxic to the larvae tested. Bacillus thuringiensis isolated appears to be more toxic to Aedes larva than Culex larva. The most effective isolate was BT5, with a more than 65 % mortality percentage. The percentage occurrence of Bacillus thuringiensis among the isolates is 62.5%. Crystal (Cry) 1 and 2 protein gene occurs in 100 and 80 % of the isolates, respectively.
Conclusion: Formulations of B. thuringiensis from the isolates could serve as a form of biopesticide on mosquitoes and consequently control malaria and other mosquito-borne diseases of global health concern. J Microbiol Infect Dis 2021; 11(4):224-232.

Key words: Biopesticide, Larvae, Toxicity, Bacillus thuringiensis, Aedes, Culex


 
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How to Cite this Article
Pubmed Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman. Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae. J Microbiol Infect Dis. 2021; 11(4): 224-232 . doi:10.5799/jmid.1036820

Web Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman. Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae. https://www.jmidonline.org/?mno=302657078 [Access: November 23, 2024]. doi:10.5799/jmid.1036820

AMA (American Medical Association) Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman. Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae. J Microbiol Infect Dis. 2021; 11(4): 224-232 . doi:10.5799/jmid.1036820


Vancouver/ICMJE Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman. Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae. J Microbiol Infect Dis. (2021), [cited November 23, 2024]; 11(4): 224-232 . doi:10.5799/jmid.1036820


Harvard Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman (2021) Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae. J Microbiol Infect Dis, 11 (4), 224-232 . doi:10.5799/jmid.1036820


Turabian Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman. 2021. Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae. Journal of Microbiology and Infectious Diseases, 11 (4), 224-232 . doi:10.5799/jmid.1036820


Chicago Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman. "Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae." Journal of Microbiology and Infectious Diseases 11 (2021), 224-232 . doi:10.5799/jmid.1036820


MLA (The Modern Language Association) Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman. "Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae." Journal of Microbiology and Infectious Diseases 11.4 (2021), 224-232 . Print. doi:10.5799/jmid.1036820


APA (American Psychological Association) Style

Majekodunmi Racheal Adedayo, Azeezat Ayodeji Uthman (2021) Bacillus thuringiensis Isolated from Flour Mill Soil And Its Toxicity Against Culex And Aedes Larvae. Journal of Microbiology and Infectious Diseases, 11 (4), 224-232 . doi:10.5799/jmid.1036820


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Journal of Microbiology and Infectious Diseases

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