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2024, Vol: 14, Issue: 3
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Research Article 


Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka.


Abstract
Objectives: Methicillin resistant Staphylococcus aureus (MRSA) nasal carriage often precedes infection. This work aims to genetically profile MRSA carriage of resistant genes (mecA, blaZ and tetM) and certain virulence genes (nuc, pvl, spa and scn).
Methods: Phenotypically confirmed Staphylococcus aureus were Screened for oxacillin resistance and assessed for β-lactamase production. Further confirmation of identity was performed by multiplex PCR of the 16SrRNA, mecA, tetM, pvl, nuc, spa and scn genes. DNA gel fingerprint analysis was carried out and the bands were clustered in a dendrogram.
Results: The study analysed 81 Staphylococcus aureus isolates for Methicillin resistance out of which 38 (46.9%) were MRSA. The DNA extraction method was effective for all MRSA isolates evaluated. All phenotypic MRSA isolates (100%) were positive for the S. aureus specific 16SrRNA gene confirming that these isolates were S. aureus strains. The nuc gene was detected in 19/38 (50%) of the MRSA isolates, blaZ gene in 14/38 (36.8%) and tetM in 18/38 (47.4%).
Conclusions: The need for improved vigilance to recognize MRSA strains cannot be overemphasized. If MRSA control is not tracked, these strains will increase exponentially. J Microbiol Infect Dis 2020; 10(3):144-153.

Key words: Methicillin resistant Staphylococcus aureus, PCR simulation, mecA, Electrophoretogram


 
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How to Cite this Article
Pubmed Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka. Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR. J Microbiol Infect Dis. 2020; 10(3): 144-153 . doi:10.5799/jmid.790286

Web Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka. Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR. https://www.jmidonline.org/?mno=302657169 [Access: November 22, 2024]. doi:10.5799/jmid.790286

AMA (American Medical Association) Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka. Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR. J Microbiol Infect Dis. 2020; 10(3): 144-153 . doi:10.5799/jmid.790286


Vancouver/ICMJE Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka. Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR. J Microbiol Infect Dis. (2020), [cited November 22, 2024]; 10(3): 144-153 . doi:10.5799/jmid.790286


Harvard Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka (2020) Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR. J Microbiol Infect Dis, 10 (3), 144-153 . doi:10.5799/jmid.790286


Turabian Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka. 2020. Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR. Journal of Microbiology and Infectious Diseases, 10 (3), 144-153 . doi:10.5799/jmid.790286


Chicago Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka. "Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR." Journal of Microbiology and Infectious Diseases 10 (2020), 144-153 . doi:10.5799/jmid.790286


MLA (The Modern Language Association) Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka. "Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR." Journal of Microbiology and Infectious Diseases 10.3 (2020), 144-153 . Print. doi:10.5799/jmid.790286


APA (American Psychological Association) Style

Suleiman Shuaibu Adeiza, Josiah Ademola Onaolapo, Busayo Olalekan Olayinka (2020) Genetic Profiling of Methicillin-resistant Staphylococcus aureus in an African Hospital by Multiplex-PCR. Journal of Microbiology and Infectious Diseases, 10 (3), 144-153 . doi:10.5799/jmid.790286


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Journal of Microbiology and Infectious Diseases

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University of Tripoli

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