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2024, Vol: 14, Issue: 3
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Research Article 


Effect of temperature on storage of ethionamide during susceptibility testing

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar.


Cited By:1

Abstract
Objectives: Ethionamide being thermolabile in nature, effect of temperature on the drug and its stability in liquid and solid media during susceptibility testing procedures was assessed to understand the inconsistency in DST formats.
Materials and Methods: Working solution of ethionamide and Lowenstein- Jensen (LJ) media incorporated with ethionamide were preincubated at 4oC and 37oC prior to DST methods was incubated till 4 weeks at different temperatures and utilized for DST in MGIT 960.
Results: Degradation of ethionamide working solution was observed at 37oC after 3 weeks of incubation. Ethionamide incorporated LJ media can be stored without compromise on susceptibility up to 5 weeks. But, a week of prior incubation at 37oC has deleterious effect on the DST profile. Ethionamide was found to degrade at 37oC after different time points when stored as solution or as LJ media.
Conclusion: Use of DST formats that provide results within 2 weeks can be recommended for ethionamide susceptibility testing.


 
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How to Cite this Article
Pubmed Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar. Effect of temperature on storage of ethionamide during susceptibility testing. J Microbiol Infect Dis. 2013; 3(3): 128-132 . doi:10.5799/jmid2013.03.0095

Web Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar. Effect of temperature on storage of ethionamide during susceptibility testing. https://www.jmidonline.org/?mno=302657132 [Access: November 22, 2024]. doi:10.5799/jmid2013.03.0095

AMA (American Medical Association) Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar. Effect of temperature on storage of ethionamide during susceptibility testing. J Microbiol Infect Dis. 2013; 3(3): 128-132 . doi:10.5799/jmid2013.03.0095


Vancouver/ICMJE Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar. Effect of temperature on storage of ethionamide during susceptibility testing. J Microbiol Infect Dis. (2013), [cited November 22, 2024]; 3(3): 128-132 . doi:10.5799/jmid2013.03.0095


Harvard Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar (2013) Effect of temperature on storage of ethionamide during susceptibility testing. J Microbiol Infect Dis, 3 (3), 128-132 . doi:10.5799/jmid2013.03.0095


Turabian Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar. 2013. Effect of temperature on storage of ethionamide during susceptibility testing. Journal of Microbiology and Infectious Diseases, 3 (3), 128-132 . doi:10.5799/jmid2013.03.0095


Chicago Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar. "Effect of temperature on storage of ethionamide during susceptibility testing." Journal of Microbiology and Infectious Diseases 3 (2013), 128-132 . doi:10.5799/jmid2013.03.0095


MLA (The Modern Language Association) Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar. "Effect of temperature on storage of ethionamide during susceptibility testing." Journal of Microbiology and Infectious Diseases 3.3 (2013), 128-132 . Print. doi:10.5799/jmid2013.03.0095


APA (American Psychological Association) Style

Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar (2013) Effect of temperature on storage of ethionamide during susceptibility testing. Journal of Microbiology and Infectious Diseases, 3 (3), 128-132 . doi:10.5799/jmid2013.03.0095


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