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2024, Vol: 14, Issue: 3
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Research Article 


Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani.


Cited By:9

Abstract
Objectives: In the present study, we have analyzed ESBL-producing S. typhi 's capability in forming a significant amount of biofilm on plastic and glass surface, and the influence of cefetoxime on biofilm development at subinhibitory (Sub-MIC) concentration.
Methods: Nine strains of cefetoxime-mediated ESBL-producing S. typhi were used in the study. S. typhi formed biofilm on plastic and glass materials; it was demonstrated using micro titre plate (MTP) and standard test tube methods. Comparative study of the influence of cefetoxime on biofilm formation in its MIC (128 µg/ml) and at sub-MIC (64 µg/ml) was demonstrated by microtitre plate method. The biofilm production was observed in SEM images, statistical analysis (ANOVA) showed significant increase in cell surface and volume due to the influence of Cefetoxime.
Results: Of the nine selected isolates, two S. typhi strains, namely BST 51 and BST 130, produced relatively strong biofilm in the presence of cefetoxime at sub-MIC level (64 µg/ml), comparatively weak biofilm formation at MIC level (128 µg/ml). Typical morphological changes were observed in cefetoxime-resistant strains, S. typhi BST 51 and BST 130, in comparison to cefetoxime-sensitive strain S. typhi BST 63 used as a control. We found an increase in surface and volume of a cell in response to cefetoxime and statistical data (ANOVA) proved that resistant strains were significantly different from control strains.
Conclusion: The above study clearly shows that cefetoxime at sub-MIC level efficiently induces biofilm formation and promotes changes in morphology of the cell. J Microbiol Infect Dis 2017; 7(2): 67-75

Key words: Salmonella typhi, Biofilm, Cefetoxime, Subinhibitory concentration (Sub-MIC), MIC, SEM


 
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How to Cite this Article
Pubmed Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani. Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi. J Microbiol Infect Dis. 2017; 7(2): 67-74 . doi:10.5799/jmid.328786

Web Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani. Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi. https://www.jmidonline.org/?mno=302657131 [Access: November 22, 2024]. doi:10.5799/jmid.328786

AMA (American Medical Association) Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani. Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi. J Microbiol Infect Dis. 2017; 7(2): 67-74 . doi:10.5799/jmid.328786


Vancouver/ICMJE Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani. Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi. J Microbiol Infect Dis. (2017), [cited November 22, 2024]; 7(2): 67-74 . doi:10.5799/jmid.328786


Harvard Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani (2017) Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi. J Microbiol Infect Dis, 7 (2), 67-74 . doi:10.5799/jmid.328786


Turabian Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani. 2017. Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi. Journal of Microbiology and Infectious Diseases, 7 (2), 67-74 . doi:10.5799/jmid.328786


Chicago Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani. "Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi." Journal of Microbiology and Infectious Diseases 7 (2017), 67-74 . doi:10.5799/jmid.328786


MLA (The Modern Language Association) Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani. "Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi." Journal of Microbiology and Infectious Diseases 7.2 (2017), 67-74 . Print. doi:10.5799/jmid.328786


APA (American Psychological Association) Style

Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli, Chandrakanth Kelmani (2017) Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi. Journal of Microbiology and Infectious Diseases, 7 (2), 67-74 . doi:10.5799/jmid.328786


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Journal of Microbiology and Infectious Diseases

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