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2024, Vol: 14, Issue: 4
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Research Article 


Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction

Nupur Gupta, Manoj Jais, Priyanshu Kumar Shrivastava, Aditi Sharma.


Abstract
Objectives: The objective of the current study was to compare the diagnostic methods of Oxacillin Disk Diffusion, Cefoxitin Disk Diffusion, Oxacillin Resistance Screening Agar Base, and CHROM Agar MRSA with the gold-standard method of Polymerase Chain Reaction for detection of Methicillin-resistant Staphylococcus aureus.
Methods: Two hundred pus samples were included in the study from which Staphylococcus strains were evaluated. The isolates of Staphylococcus aureus were subjected to the Oxacillin Disk Diffusion test, Cefoxitin Disk Diffusion test, Oxacillin Resistance Screening Agar Base, and CHROM Agar MRSA to detect MRSA with PCR, the reference standard. The diagnostic techniques were compared to their sensitivity, specificity, positive predictive, and negative predictive values.
Results: The sensitivity of the Cefoxitin Disk Diffusion test was 100%, followed by CHROM Agar MRSA at 96.7%, Oxacillin Disk Diffusion at 90%, and Oxacillin Resistance Screening Agar Base at 86.7%. Most specific was the Cefoxitin Disk Diffusion test (99.4%), followed by Oxacillin Resistance Screening Agar Base (98.8%), CHROM Agar MRSA (97.7%), and the least specific was the Oxacillin Disk Diffusion test (96.5%).
Conclusion: The Cefoxitin Disk Diffusion test was the most sensitive and specific of all four methods, next to the Polymerase Chain Reaction. However, future multicentric studies are recommended to test this method across all prevalent centers of methicillin resistance. J Microbiol Infect Dis 2022; 12(3):116-126.

Key words: CHROM Agar, Methicillin-Resistant Staphylococcus aureus, Oxacillin, Cefoxitin, Polymerase Chain Reaction


 
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How to Cite this Article
Pubmed Style

Gupta N, Jais M, Shrivastava PK, Sharma A. Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction. J Microbiol Infect Dis. 2022; 12(3): 116-126 . doi:10.5799/jmid.1176537

Web Style

Gupta N, Jais M, Shrivastava PK, Sharma A. Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction. https://www.jmidonline.org/?mno=302657113 [Access: January 09, 2025]. doi:10.5799/jmid.1176537

AMA (American Medical Association) Style

Gupta N, Jais M, Shrivastava PK, Sharma A. Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction. J Microbiol Infect Dis. 2022; 12(3): 116-126 . doi:10.5799/jmid.1176537


Vancouver/ICMJE Style

Gupta N, Jais M, Shrivastava PK, Sharma A. Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction. J Microbiol Infect Dis. (2022), [cited January 09, 2025]; 12(3): 116-126 . doi:10.5799/jmid.1176537


Harvard Style

Gupta, N., Jais, . M., Shrivastava, . P. K. & Sharma, . A. (2022) Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction. J Microbiol Infect Dis, 12 (3), 116-126 . doi:10.5799/jmid.1176537


Turabian Style

Gupta, Nupur, Manoj Jais, Priyanshu Kumar Shrivastava, and Aditi Sharma. 2022. Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction. Journal of Microbiology and Infectious Diseases, 12 (3), 116-126 . doi:10.5799/jmid.1176537


Chicago Style

Gupta, Nupur, Manoj Jais, Priyanshu Kumar Shrivastava, and Aditi Sharma. "Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction." Journal of Microbiology and Infectious Diseases 12 (2022), 116-126 . doi:10.5799/jmid.1176537


MLA (The Modern Language Association) Style

Gupta, Nupur, Manoj Jais, Priyanshu Kumar Shrivastava, and Aditi Sharma. "Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction." Journal of Microbiology and Infectious Diseases 12.3 (2022), 116-126 . Print. doi:10.5799/jmid.1176537


APA (American Psychological Association) Style

Gupta, N., Jais, . M., Shrivastava, . P. K. & Sharma, . A. (2022) Comparison of Different Phenotypic Methods of Detection of Methicillin-Resistant Staphylococcus aureus with Polymerase Chain Reaction. Journal of Microbiology and Infectious Diseases, 12 (3), 116-126 . doi:10.5799/jmid.1176537


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