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2024, Vol: 14, Issue: 4
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Research Article 


Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital

Kang Liao, Yili Chen, Han Huang, Penghao Guo, Dongmei Chen, Min Liu, Yan Zeng.


Cited By:2

Abstract
Objective: The aim of this study was to investigate the molecular characteristics of carbapenem-resistant Enterobacteria¬ceae (CRE) isolates from a teaching hospital in Guangdong, China, during the period between January 2008 and December 2011.
Methods: A total of 28 (0.9%) CRE isolates were collected among 3286 isolates. Antimicrobial susceptibility testing, Modified Hodge Test (MHT) and genotyping by pulsed-field gel electrophoresis were performed. Extended-spectrum β-lactamase (ESBL) and carbapenemase genes were detected using gene amplification and sequencing. Outer membrane porin (OMP) genes (OmpK35/OmpK36 for K. pneumoniae, OmpC/OmpF for E. cloacae were examined.
Results: Seven (25.0%) isolates of the 28 carbapenem resistant Enterobacteriaceae had a positive MHT. PCR and sequenc¬ing analysis revealed that 19 of the 28 isolates were molecularly confirmed to harbor ESBLs (15 isolates, 53.6%), AmpC (6 isolates, 21.4%), or IMP-4 (five isolates, 17.9%), and nine (32.1%) isolates of the 28 isolates possessed at least two different classes of β-lactamases. Specially, five (71.4%) of seven MHT-positive CRE isolates is due to the production of the carbapen¬emase-encoding gene IMP-4. Almost all of the CRE isolates in the present study loss gene expressions of OmpK35 and/or OmpK36 in K. pneumoniae, OmpC and/or OmpF in E. cloacae, except for one K. pneumoniae. PFGE analysis demonstrated genomic variability.
Conclusion: Carbapenem resistance in Enterobacteriaceae is due to the combined effect of β- lactamases with porin im¬permeability caused by loss of OMPs observed in these organisms. To prevent both CRE transmission and infections has become significantly urgent public health objectives. J Microbiol Infect Dis 2014; 4(4): 145-151

Key words: Molecular characteristics, Carbapenem-resistant, Enterobacteriaceae, IMP-4. Kang Liao, Yili Chen, Han Huang, Penghao Guo, Dongmei Chen, Min Liu, Yan Zeng. Molecular Characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital in Guangdong. J Microbiol Infect Dis 2014; 4(4): 145-151 doi: 10.5799/ahinjs.02.2014.04.0158


 
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How to Cite this Article
Pubmed Style

Liao K, Chen Y, Huang H, Guo P, Chen D, Liu M, Zeng Y. Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital. J Microbiol Infect Dis. 2014; 4(4): 145-151 . doi:10.5799/jmid2014.04.0158

Web Style

Liao K, Chen Y, Huang H, Guo P, Chen D, Liu M, Zeng Y. Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital. https://www.jmidonline.org/?mno=302657070 [Access: January 09, 2025]. doi:10.5799/jmid2014.04.0158

AMA (American Medical Association) Style

Liao K, Chen Y, Huang H, Guo P, Chen D, Liu M, Zeng Y. Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital. J Microbiol Infect Dis. 2014; 4(4): 145-151 . doi:10.5799/jmid2014.04.0158


Vancouver/ICMJE Style

Liao K, Chen Y, Huang H, Guo P, Chen D, Liu M, Zeng Y. Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital. J Microbiol Infect Dis. (2014), [cited January 09, 2025]; 4(4): 145-151 . doi:10.5799/jmid2014.04.0158


Harvard Style

Liao, K., Chen, . Y., Huang, . H., Guo, . P., Chen, . D., Liu, . M. & Zeng, . Y. (2014) Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital. J Microbiol Infect Dis, 4 (4), 145-151 . doi:10.5799/jmid2014.04.0158


Turabian Style

Liao, Kang, Yili Chen, Han Huang, Penghao Guo, Dongmei Chen, Min Liu, and Yan Zeng. 2014. Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital. Journal of Microbiology and Infectious Diseases, 4 (4), 145-151 . doi:10.5799/jmid2014.04.0158


Chicago Style

Liao, Kang, Yili Chen, Han Huang, Penghao Guo, Dongmei Chen, Min Liu, and Yan Zeng. "Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital." Journal of Microbiology and Infectious Diseases 4 (2014), 145-151 . doi:10.5799/jmid2014.04.0158


MLA (The Modern Language Association) Style

Liao, Kang, Yili Chen, Han Huang, Penghao Guo, Dongmei Chen, Min Liu, and Yan Zeng. "Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital." Journal of Microbiology and Infectious Diseases 4.4 (2014), 145-151 . Print. doi:10.5799/jmid2014.04.0158


APA (American Psychological Association) Style

Liao, K., Chen, . Y., Huang, . H., Guo, . P., Chen, . D., Liu, . M. & Zeng, . Y. (2014) Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary Hospital. Journal of Microbiology and Infectious Diseases, 4 (4), 145-151 . doi:10.5799/jmid2014.04.0158


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