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2024, Vol: 14, Issue: 4
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Research Article 


Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach

Hind Abdelrahman Hassan, Khoubieb Ali Abd-elrahman, Nasr Mohammed Nasr, Yassir A. Almofti.


Cited By:1

Abstract
Objectives: Yellow fever virus (YFV) is an enveloped positive sense RNA virus. It is the causative agent of the mosquito-borne disease yellow fever. The aim of this study was to design multi epitopes vaccine for YFV from envelope protein eliciting humoral and cellular immunity.
Methods: Twenty six YFV strains envelope proteins were retrieved from NCBI. The immune epitope database analysis resources (IEDB) were used for epitopes prediction.
Results: Eleven epitopes successfully passed all B cell prediction tools, among them four epitopes 33VMAPDKPSL41, 72DKCP77, 236PPHA239 and 385LTYQ388 demonstrated higher score in Emini and Kolaskar and tongaonker software. Thus were proposed as B cells epitopes. For T cells; 28 epitopes interacted with MHC-I and the best recognizable epitopes were 471MTMSMSMIL479, 363VLIEVNPPF371, 33VMAPDKPSL41 and 226REMHHLVEF234. For MHC-II ninety epitopes were predicted and the best epitopes were 284RVKLSALTL292, 363VLIEVNPPF371, 479LVGVIMMFL487 and 226REMHHLVEF234. Strikingly the epitope 33VMAPDKPSL41 successfully interacted with both B and T cells. Also 363VLIEVNPPF371 and 226REMHHLVEF234 demonstrated successful interaction with T cells. The population coverage was 84.66% and 99.91% for MHC-I and MHC-II epitopes, respectively, and 99.99% for all T cells epitopes.
Conclusions: Taken together nine epitopes successfully proposed as vaccine candidate against YFV. In vivo and in vitro clinical trials studies are required to elucidate the effectiveness of these epitopes as vaccine. J Microbiol Infect Dis 2020; 10(1):31-46.

Key words: Yellow fever virus; NCBI; IEDB; Immunoinformatics; Insilico vaccine


 
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How to Cite this Article
Pubmed Style

Hassan HA, Abd-elrahman KA, Nasr NM, Almofti YA. Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach. J Microbiol Infect Dis. 2020; 10(1): 31-46 . doi:10.5799/jmid.700510

Web Style

Hassan HA, Abd-elrahman KA, Nasr NM, Almofti YA. Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach. https://www.jmidonline.org/?mno=302657056 [Access: January 09, 2025]. doi:10.5799/jmid.700510

AMA (American Medical Association) Style

Hassan HA, Abd-elrahman KA, Nasr NM, Almofti YA. Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach. J Microbiol Infect Dis. 2020; 10(1): 31-46 . doi:10.5799/jmid.700510


Vancouver/ICMJE Style

Hassan HA, Abd-elrahman KA, Nasr NM, Almofti YA. Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach. J Microbiol Infect Dis. (2020), [cited January 09, 2025]; 10(1): 31-46 . doi:10.5799/jmid.700510


Harvard Style

Hassan, H. A., Abd-elrahman, . K. A., Nasr, . N. M. & Almofti, . Y. A. (2020) Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach. J Microbiol Infect Dis, 10 (1), 31-46 . doi:10.5799/jmid.700510


Turabian Style

Hassan, Hind Abdelrahman, Khoubieb Ali Abd-elrahman, Nasr Mohammed Nasr, and Yassir A. Almofti. 2020. Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach. Journal of Microbiology and Infectious Diseases, 10 (1), 31-46 . doi:10.5799/jmid.700510


Chicago Style

Hassan, Hind Abdelrahman, Khoubieb Ali Abd-elrahman, Nasr Mohammed Nasr, and Yassir A. Almofti. "Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach." Journal of Microbiology and Infectious Diseases 10 (2020), 31-46 . doi:10.5799/jmid.700510


MLA (The Modern Language Association) Style

Hassan, Hind Abdelrahman, Khoubieb Ali Abd-elrahman, Nasr Mohammed Nasr, and Yassir A. Almofti. "Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach." Journal of Microbiology and Infectious Diseases 10.1 (2020), 31-46 . Print. doi:10.5799/jmid.700510


APA (American Psychological Association) Style

Hassan, H. A., Abd-elrahman, . K. A., Nasr, . N. M. & Almofti, . Y. A. (2020) Identification of Novel Vaccine Candidates against Yellow Fever Virus from the Envelope Protein: An insilico Approach. Journal of Microbiology and Infectious Diseases, 10 (1), 31-46 . doi:10.5799/jmid.700510


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