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Research Article 


Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya.


Abstract
Objectives: The study was aimed to evaluate the roles of Lactobacillus acidophilus supplementation in maintaining intestinal epithelial integrities, tight junction proteins, and adhesion molecules in non-specific diarrhea.
Methods: In this study, we used the pre-weaned BL/6 pups (3 weeks of age, same litters) as a model animal. We supplied the non-sterilized and poor-quality water to experimental pups (n=7) to develop non-specific diarrheic symptoms. Then diarrheic pups were supplemented with Lactobacillus acidophilus (LA) for three consecutive weeks. The control group (n=5) was supplied with sterilized water and no LA. The sampling and analysis were performed on day 0, day 7, day 14, and day 21. The expressions of pro-inflammatory cytokines (IL-6, TNF-a) and tight junction proteins (TJPs) of gut mucosa were determined using qRT-PCR. And the serum cytokines level was screened through sandwich ELISA.
Results: The intestinal cytoskeletal integrity becomes disrupted and characterized by lower ZO-1, Occludin, Claudin-1, Claudin-5, and JAM mRNA expressions upon real-time qRT-PCR. However, Claudin-4 was found to be not affected and illustrated with a higher expression like control pups. Interestingly, supplementing Lactobacillus acidophilus was found to maintain gut integrity and effectively reduce diarrheic symptoms. Like the control pups, the Lactobacillus acidophilus supplemented pups exhibited a higher expression of gut epithelial TJPs and adhesion molecules. Moreover, the diseased pups produced significantly increased IL-6, and TNF-α production in blood serum, compared to control BL/6 pups.
Conclusion: We concluded that L. acidophilus supplementation might orchestrate the equilibrium of gut health and immunity against non-specific diarrhea. J Microbiol Infect Dis 2022; 12(2):54-62.


 
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How to Cite this Article
Pubmed Style

Hossain FMA, Khan P, Zahran I, Atif SM, Zinnah MA, Khan IU, Rahman MM, Sumaiya FI. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis. 2022; 12(2): 54-62 . doi:10.5799/jmid.1130109

Web Style

Hossain FMA, Khan P, Zahran I, Atif SM, Zinnah MA, Khan IU, Rahman MM, Sumaiya FI. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. https://www.jmidonline.org/?mno=302657032 [Access: January 09, 2025]. doi:10.5799/jmid.1130109

AMA (American Medical Association) Style

Hossain FMA, Khan P, Zahran I, Atif SM, Zinnah MA, Khan IU, Rahman MM, Sumaiya FI. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis. 2022; 12(2): 54-62 . doi:10.5799/jmid.1130109


Vancouver/ICMJE Style

Hossain FMA, Khan P, Zahran I, Atif SM, Zinnah MA, Khan IU, Rahman MM, Sumaiya FI. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis. (2022), [cited January 09, 2025]; 12(2): 54-62 . doi:10.5799/jmid.1130109


Harvard Style

Hossain, F. M. A., Khan, . P., Zahran, . I., Atif, . S. M., Zinnah, . M. A., Khan, . I. U., Rahman, . M. M. & Sumaiya, . F. I. (2022) Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis, 12 (2), 54-62 . doi:10.5799/jmid.1130109


Turabian Style

Hossain, Ferdaus Mohd Altaf, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, and Farzana Islam Sumaiya. 2022. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. Journal of Microbiology and Infectious Diseases, 12 (2), 54-62 . doi:10.5799/jmid.1130109


Chicago Style

Hossain, Ferdaus Mohd Altaf, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, and Farzana Islam Sumaiya. "Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea." Journal of Microbiology and Infectious Diseases 12 (2022), 54-62 . doi:10.5799/jmid.1130109


MLA (The Modern Language Association) Style

Hossain, Ferdaus Mohd Altaf, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, and Farzana Islam Sumaiya. "Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea." Journal of Microbiology and Infectious Diseases 12.2 (2022), 54-62 . Print. doi:10.5799/jmid.1130109


APA (American Psychological Association) Style

Hossain, F. M. A., Khan, . P., Zahran, . I., Atif, . S. M., Zinnah, . M. A., Khan, . I. U., Rahman, . M. M. & Sumaiya, . F. I. (2022) Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. Journal of Microbiology and Infectious Diseases, 12 (2), 54-62 . doi:10.5799/jmid.1130109


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