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Research Article 


Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome

Arindam Chakraborty, Prabha Adhikari, Shalini Shenoy, Shrikala Baliga, Satish Rao, Dhanashree B, Vishwas Saralaya.


Cited By:11

Abstract
Objective: We investigated the expression of Extended Spectrum β-Lactamases (ESBLs), AmpC β lactamases and Carbapenemases in extraintestinal pathogenic Escherichia coli (ExPEC) isolates and correlated with treatment and outcome of the patients.
Methods: Three hundred ExPEC infected patients were included in the study. Demographic data, antibiogram, treatment and outcome were collected. Production of ESBLs was detected by combination disk method; AmpC was detected by AmpC disk test. Carbapenemase production was detected by disk diffusion and confirmed by modified Hodge test. Identification of metallo- β-lactamase (MBL) activity was performed by the carbapenem-EDTA combined disk method and MBL E-test.
Results: Out of 300 E. coli isolates, 212 (71%) were ESBL producers. AmpC β lactamase production was seen in 95 (32%) isolates; 16 (17%) isolates were pure AmpC producers whereas 79 (83%) were ESBL co-producers. Twenty nine (9.5%) isolates were carbapenemase producers of which 15 (5%) were MBL producers. For treatment, most widely prescribed antibiotics were β-lactam+β-lactamase inhibitor combinations (39%). Sixty seven percent patients improved; relapse/re-infection was seen in 18% of patients and 11% patients expired. Increased mortality was seen in patients with blood stream infection and more number of relapses was seen in urinary tract infection.
Conclusion: ExPEC producing ESBL or AmpC along with carbapenemases are particularly challenging for clinicians and are a major threat worldwide. Early use of appropriate antibiotics like β-lactam+β-lactamase inhibitor combinations will probably reduce complications in these patients.


 
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How to Cite this Article
Pubmed Style

Chakraborty A, Adhikari P, Shenoy S, Baliga S, Rao S, B D, Saralaya V. Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome. J Microbiol Infect Dis. 2013; 3(4): 150-156 . doi:10.5799/jmid2013.04.0100

Web Style

Chakraborty A, Adhikari P, Shenoy S, Baliga S, Rao S, B D, Saralaya V. Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome. https://www.jmidonline.org/?mno=302656968 [Access: January 09, 2025]. doi:10.5799/jmid2013.04.0100

AMA (American Medical Association) Style

Chakraborty A, Adhikari P, Shenoy S, Baliga S, Rao S, B D, Saralaya V. Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome. J Microbiol Infect Dis. 2013; 3(4): 150-156 . doi:10.5799/jmid2013.04.0100


Vancouver/ICMJE Style

Chakraborty A, Adhikari P, Shenoy S, Baliga S, Rao S, B D, Saralaya V. Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome. J Microbiol Infect Dis. (2013), [cited January 09, 2025]; 3(4): 150-156 . doi:10.5799/jmid2013.04.0100


Harvard Style

Chakraborty, A., Adhikari, . P., Shenoy, . S., Baliga, . S., Rao, . S., B, . D. & Saralaya, . V. (2013) Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome. J Microbiol Infect Dis, 3 (4), 150-156 . doi:10.5799/jmid2013.04.0100


Turabian Style

Chakraborty, Arindam, Prabha Adhikari, Shalini Shenoy, Shrikala Baliga, Satish Rao, Dhanashree B, and Vishwas Saralaya. 2013. Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome. Journal of Microbiology and Infectious Diseases, 3 (4), 150-156 . doi:10.5799/jmid2013.04.0100


Chicago Style

Chakraborty, Arindam, Prabha Adhikari, Shalini Shenoy, Shrikala Baliga, Satish Rao, Dhanashree B, and Vishwas Saralaya. "Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome." Journal of Microbiology and Infectious Diseases 3 (2013), 150-156 . doi:10.5799/jmid2013.04.0100


MLA (The Modern Language Association) Style

Chakraborty, Arindam, Prabha Adhikari, Shalini Shenoy, Shrikala Baliga, Satish Rao, Dhanashree B, and Vishwas Saralaya. "Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome." Journal of Microbiology and Infectious Diseases 3.4 (2013), 150-156 . Print. doi:10.5799/jmid2013.04.0100


APA (American Psychological Association) Style

Chakraborty, A., Adhikari, . P., Shenoy, . S., Baliga, . S., Rao, . S., B, . D. & Saralaya, . V. (2013) Expression of ESBL, MBL and AmpC B lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome. Journal of Microbiology and Infectious Diseases, 3 (4), 150-156 . doi:10.5799/jmid2013.04.0100


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